Transient expression of DNA and RNA in parasitic helminths by using particle bombardment
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چکیده
منابع مشابه
Transient expression of DNA and RNA in parasitic helminths by using particle bombardment.
Parasitic helminths (worms belonging to several metazoan phyla) cause considerable morbidity and mortality in humans. They are an important veterinary problem, and they result in significant economic losses in animal grazing and agriculture. Experimental studies on parasitic helminths have been limited by a lack of parasite cell lines and methods for molecular genetic analyses. We evaluated par...
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Transfection of trypanosomatids, particularly the transfection of their mitochondrion and different developmental stages, is still a limiting step in many experiments using recombinant DNA technology. Two types of trypanosomatid transfection protocols have been described so far. The standard protocol is based on electroporation first described by Bellofatto and Cross [1]. The second protocol is...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1999
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.96.15.8687